A Comparison of Two Media Formulations and Two Vented Culture Vessels for Shoot Multiplication and Rooting of Hemp Shoot Tip Cultures

Micropropagation of hemp ( Cannabis sativa ) is constrained by problems with hyperhydricity and culture decline microshoots. These can be reduced increasing agar nutrients in the media during micropropagation stages 1 2, respectfully. Performance microshoots ‘Abacus’ ‘Wife’ cultured Driver Kuniyuki Walnut medium (DKW) for 15 weeks (6 stage + 9 2), subculturing every 3 both or Murashige Skoog vitamins (MS) 6 (stage 1) followed Lubell-Brand (LBC) was evaluated. In a separate study, microshoot performance MS LBC weeks, using boxes (Magenta GA-7) lids featuring vent diameter 10 mm pore size 0.2 µM microboxes (Sac O 2 O95/114 OD95) filter #10) were Shoot multiplication rate (SMR) explant height greater than DKW. For ‘Wife’, SMR at LBC, as provided more water cultures had received initially 1. Culture did not influence ex vitro rooting success, which 75% ≥ 90% ‘Wife’. Microboxes resulted shoots lower percentage boxes. cultivars that are highly prone to developing hyperhydricity, like ‘Abacus’, adequate control this condition.